Beta actin antibody cell signaling

Results for "beta Actin" | Abcam: antibodies, proteins, kits.
Beta Actin antibody (HRP-60008) | Proteintech.
Beta Actin Antibody, HRP (HRP-60008) - Thermo Fisher Scientific.
Beta-Actin's Role in Neuronal Plasticity | Antibody News: Novus Biologicals.
Beta Actin antibody (66009-1-Ig) | Proteintech.
Beta-Actin Antibody (NB600-532): Novus Biologicals.
Recombinant Anti-beta Actin antibody [EPR21241] (ab213262).
Beta Actin Antibody - Affinity Biosciences.
Anti-Actin Antibody (C-2) | SCBT - Santa Cruz Biotechnology.
Pan-Actin Antibody | Cell Signaling Technology.
Beta Actin Antibody, HRP (A00730-100).
Beta-Actin Antibody (NB100-56874): Novus Biologicals.
Anti-beta Actin antibody [BA3R] (ARG62346) - arigo Biolaboratories.
Cell Signaling Technology beta-Actin Antibody 100 µl | Fisher.

Results for "beta Actin" | Abcam: antibodies, proteins, kits.

Antigen-antibody complexes were formed by incubating 750 µg of HeLa whole cell lysates with 2 µg of a beta-actin polyclonal antibody (Product # PA1-183) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample. The beta-Actin Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to Actb. This antibody recognizes Human, Mouse, Rat, Bovine, Drosophila/Arthropod, Hamster, Non-Human Primate, Zebrafish/Fish,.

Beta Actin antibody (HRP-60008) | Proteintech.

A locked padlock) or means you've safely connected to the website. Share sensitive information only on official, secure websites.. The most cited anti-beta-actin antibody clone is AC-15, which was used in Western blot, for example, in order to normalize Western blot signals for quantitative measurements [] and intein-mediated protein trans-splicing [].Its specificity has been confirmed in knockout studies [16, 17].Other commonly used clones include AC-74 (MilliporeSigma A5316 [] and A2228 [] ) and C4 (SCBT sc-47778.

Beta Actin Antibody, HRP (HRP-60008) - Thermo Fisher Scientific.

Preparation. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin / ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Beta-Actin's Role in Neuronal Plasticity | Antibody News: Novus Biologicals.

Shop Cell Signaling Technology beta-Actin Antibody 100 µl at F Fisher Scientific... beta-Actin Antibody 100 µl. Catalog No. 50-192-760. $355.88 / Each; Qty. FC experiment of A431 using CL594-66009. 1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Beta Actin (CL594-66009, Clone:2D4H5) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). View All Images (5). Description: beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting.

Beta Actin antibody (66009-1-Ig) | Proteintech.

Β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) Cell Signaling Technology catalog: 8584. Beta actin antibody detects beta actin protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta actin antibody (GTX124214) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min. This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc). If this antibody is used as a loading control in WB and if the signal of the gamma actin is close to the protein of interest to be detected, we recommend cutting the membrane between the expected signals of beta actin and gamma actin.

Beta-Actin Antibody (NB600-532): Novus Biologicals.

Beta Actin Antibody (MA1-91399) in ICC/IF. Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. Mouse Anti-ß-Actin Monoclonal Antibody. Jamal, M.H. et al. (2020). Rapamycin treatment correlates changes in primary cilia expression with cell cycle regulation in epithelial cells. Biochem Pharmacol. doi: 10.1016/ Sherpa, R.T. et al. (2019). Sensory primary cilium is a responsive cAMP microdomain in renal epithelia.

Recombinant Anti-beta Actin antibody [EPR21241] (ab213262).

Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously. Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein.

Beta Actin Antibody - Affinity Biosciences.

. Beta Actin Antibody (MA1-140) in ICC/IF. Immunofluorescent analysis of Beta- Actin (green) in U2OS cells untreated or treated with 1uM Actinomycin D for 20 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature.

Anti-Actin Antibody (C-2) | SCBT - Santa Cruz Biotechnology.

ACTB (β-actin) is crucial for organogenesis, especially for the development of brain, kidney and heart. It is responsible for maintaining cell proliferation, migration and shape. Actin protein is essential for the formation of mature platelets. β−Actin plays a key role in embryonic development. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.

Pan-Actin Antibody | Cell Signaling Technology.

Proteintech's Mouse Monoclonal Beta Actin antibody is validated in FC and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey, dog samples.... Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the. Anti-beta Actin antibody [SP124] (ab115777) Research with confidence - consistent and reproducible results with every batch. Long-term and scalable supply - powered by recombinant technology for fast production. Success from the first experiment - confirmed specificity through extensive validation. Ethical standards compliant. Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Three different human cancer cell lines (SW780, BT-20, and CLS439) were probed with the antibody. Image from verified customer review. Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of MCF-7 cells using anti-Beta Actin mAb (right) and negative control (left).

Beta Actin Antibody, HRP (A00730-100).

In humans, three main actin groups have been identified - alpha, beta, and gamma. The alpha actins are expressed primarily in skeletal, cardiac, and smooth muscle, while the remaining beta and gamma. Actin filaments provide internal mechanical support to maintain cell shape, as well as the force to drive cell movements and intracellular transport. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team. Beta-Actin Antibody from Cell Signaling Technology. Be the first to write a review! β-Actin Antibody detects endogenous levels of β-actin. Due to the high sequence identity between the actin isoforms, β-Actin Antibody may cross-react with other actin isoforms.

Beta-Actin Antibody (NB100-56874): Novus Biologicals.

Beta-Actin (13E5) Rabbit mAb 100 µl. Catalog No. 50-190-410. $355.88 / Each of 1. Qty Check Availability. Add to cart.

Anti-beta Actin antibody [BA3R] (ARG62346) - arigo Biolaboratories.

Cell Signaling Technology beta-Actin Antibody 100 µl | Fisher.

This antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. Get better batch-to-batch reproducibility with a recombinant antibody. Anti-beta Actin antibody [SP124] (ab115777) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity. WB analysis of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates in NETN lysis buffer using GTX30632 beta Actin antibody. Loading 50μg Dilution 1 μg/ml.

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